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en:processing:анализ_выделенных_областей [2012/02/07 14:24] Анна Протопопова |
en:processing:анализ_выделенных_областей [2012/02/07 15:01] Анна Протопопова |
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| {{:processing:selected_area_analysis_2.jpg|An AFM image of mica surface with three protein spheres on it}} | {{:processing:selected_area_analysis_2.jpg|An AFM image of mica surface with three protein spheres on it}} | ||
| {{:processing:selected_area_analysis_1.jpg|The table of parameters}} | {{:processing:selected_area_analysis_1.jpg|The table of parameters}} | ||
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| + | Let's understand how the parameters are calculated, starting with the objects surface area. | ||
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| + | An algorithm of surface area determination is based on triangulate meshwork constitution. The surface area is defined as a sum of triangles in selection: | ||
| + | * every 4 neighboring pixels of the image are combined in the unit cell used in the construction of a triangulated mesh, | ||
| + | * a new point located on the medium height is added at the center of every unit cell, | ||
| + | * thus four triangles are formed with one common apex at the center of the square and the other two at the sites of the unit cell, | ||
| + | * surface area is calculated as the sum of the areas of triangles, as shown in a Figure: | ||
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| + | {{:processing:триангуляция.jpg?200|}} | ||
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| + | The surface area is written into the second column of the parameters table **//S_full//**. | ||
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